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- Action spectrum - Wikipedia
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- 5 Differences between Absorption Spectrum and Action Spectrum
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An action spectrum is a graph of the rate of biological effectiveness plotted against wavelength of light. It is related to absorption spectrum in many systems. Mathematically, it describes the inverse quantity of light required to evoke a constant response. It is very rare for an action spectrum to describe the level of biological activity, since biological responses are often nonlinear with intensity.
Action spectra are typically written as unit-less responses with peak response of one, and it is also important to distinguish if an action spectrum refers to quanta at each wavelength (mol or log-photons), or to spectral power (W).
It shows which wavelength of light is most effectively used in a specific chemical reaction. Some reactants are able to use specific wavelengths of light more effectively to complete their reactions. For example, chlorophyll is much more efficient at using the red and blue regions than the green region of the light spectrum to carry out photosynthesis. Therefore, the action spectrum graph would show spikes above the wavelengths representing the colours red and blue.
The first action spectrum was made by T. W. Engelmann, who split light into its components by the prism and then illuminated Cladophora placed in a suspension of aerobic bacteria. He found that bacteria accumulated in the region of blue and red light of the split spectrum. He thus discovered the effect of the different wavelengths of light on photosynthesis and plotted the first action spectrum of photosynthesis.
Action spectra have a wide variety of uses in biological and chemical research, particularly in understanding the effect of ultraviolet (UV) light on biological molecules and systems. UV light wavelengths range between 295 nm-400 nm and are known to induce skin and DNA damage. As a result, action spectra have been used to measure the efficiency of different light wavelengths in disinfecting water, the rate and mechanism of photodegradation of folic acid in the blood, and the chirality of molecules to determine secondary structure. Further examples include suppression of melatonin by wavelength and a variety of hazard functions, related to tissue damage from visible and near-visible light.
See also
Photosynthetically active radiation
Photosynthesis
Absorption spectrum
Chlorophyll a
References
External links
Plant Physiology Online: Principles of Spectrophotometry
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Action spectrum - Wikipedia
An action spectrum is a graph of the rate of biological effectiveness plotted against wavelength of light. [1] It is related to absorption spectrum in many systems. Mathematically, it describes the inverse quantity of light required to evoke a constant response.
Absorption Spectra & Action Spectra | Cambridge (CIE) A Level ...
Dec 11, 2024 · An action spectrum is a graph that shows the rate of photosynthesis at different wavelengths of light. The rate of photosynthesis is highest at the blue-violet and red regions of the light spectrum, as these are the wavelengths of light that plants can absorb (i.e. the wavelengths of light that chlorophylls and carotenoids can absorb)
Absorption Spectrum Vs Action Spectrum: Differences Explained
Aug 18, 2023 · What Is An Action Spectrum? The action spectrum refers to the wavelengths of electromagnetic radiation (i.e. light) that are most effective for photosynthesis. In other words, it describes the colors of light that are doing the actual work, …
Action Spectra - BioNinja
The action spectrum indicates the overall rate of photosynthesis at each wavelength of light The rate of photosynthesis can be measured by either the rate of carbon dioxide consumption or the level of oxygen production
Action Spectrum - an overview | ScienceDirect Topics
An action spectrum describes the degree of photoinactivation as a function of the wavelength of incident light. The commonly used parameters to describe degree of photoinactivation are plotted on the vertical axis while the horizontal axis is expressed in wavelength units.
5 Differences between Absorption Spectrum and Action Spectrum
Nov 5, 2016 · Absorption Spectrum is the graphic representation of the different wavelengths of light absorbed by the different pigments in a leaf during photosynthesis Action Spectrum is the graphic representation of the effectiveness of different wavelengths of light in photosynthesis
Absorption Spectrum vs. Action Spectrum - What's the ...
The action spectrum, also known as the effectiveness spectrum, describes the relative effectiveness of different wavelengths of light in driving a specific biological or chemical process. It represents the response or action of a biological system to different wavelengths of light.