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      ER/K-link—Leveraging a native protein linker to probe dynamic …

      Jan 1, 2021 · ER/K-linked sensors have been engineered in commonly used vectors for protein expression and purification including pET47b and pBiex1 that allow for expression and purification from bacteria and Sf9 cells.

      ERK signalling: a master regulator of cell behaviour, life and fate

      Jun 23, 2020 · In this Review, we discuss the current understanding of this important class of kinases. We begin with a brief overview of the structure, regulation, substrate recognition and subcellular...

      Here we review recent advances on the structure, stability and function of ER/K α-helices as linkers (ER/K linkers) in native proteins. We describe methodological considerations in the molecular cloning, protein expression and measurement of interaction strengths, using sensors incorporating ER/K linkers.

      Extracellular Signal-Regulated Kinase: A Regulator of Cell Growth ...

      Extracellular signal-regulated kinase (ERK) is a member of the mitogen-activated protein kinase family of signaling molecules. ERK is predominantly found in two forms, ERK1 (p44) and ERK2 (p42), respectively. There are also several atypical forms of …

      ER/K-link—Leveraging a native protein linker to probe dynamic …

      Jan 1, 2021 · The application of ER/K-linked sensors has provided numerous insights into protein interactions underlying signal transduction. Here, we highlight distinct features of ER/K-linked sensors that have been used to circumvent the limitations of traditional biophysical and cell biological techniques.

      Harnessing the unique structural properties of isolated α-helices

      Sep 12, 2014 · The ER/K α-helix, also termed the ER/K linker, has been extensively characterized in the context of the myosin family of molecular motors and is emerging as a versatile structural element for protein and cellular engineering applications.

      The extracellular signal-regulated kinase (ERK) pathway: a …

      Extracellular signal-regulated kinase (ERK) is a member of the mitogen-activated protein kinase family that is involved in both vasoconstriction and vascular smooth muscle cell growth and this, therefore, makes it attractive therapeutic target for treatment of hypertension.

      ERK signalling: a master regulator of cell behaviour, life and fate

      We begin with a brief overview of the structure, regulation, substrate recognition and subcellular localization of ERK1 and ERK2. We then systematically discuss how ERK signalling regulates six fundamental cellular processes in response to extracellular cues.

      ER/K linked GPCR-G protein fusions systematically modulate …

      Aug 10, 2017 · In this study, we describe and characterize novel sensors based on the Systematic Protein Affinity Strength Modulation (SPASM) technique. Sensors consist of a GPCR and G protein tethered by an...

      Systematic control of protein interaction using a modular ER/K α …

      Dec 12, 2011 · Our studies demonstrate the use of the ER/K α-helix to systematically engineer FRET biosensors that detect changes in concentration or affinity of interacting proteins, and modulate enzyme autoinhibition.