Jan 1, 2021 · ER/K-linked sensors have been engineered in commonly used vectors for protein expression and purification including pET47b and pBiex1 that allow for expression and …
Jun 23, 2020 · In this Review, we discuss the current understanding of this important class of kinases. We begin with a brief overview of the structure, regulation, substrate recognition and …
Here we review recent advances on the structure, stability and function of ER/K α-helices as linkers (ER/K linkers) in native proteins. We describe methodological considerations in the …
Extracellular signal-regulated kinase (ERK) is a member of the mitogen-activated protein kinase family of signaling molecules. ERK is predominantly found in two forms, ERK1 (p44) and ERK2 …
Jan 1, 2021 · The application of ER/K-linked sensors has provided numerous insights into protein interactions underlying signal transduction. Here, we highlight distinct features of ER/K-linked …
Sep 12, 2014 · The ER/K α-helix, also termed the ER/K linker, has been extensively characterized in the context of the myosin family of molecular motors and is emerging as a versatile …
Extracellular signal-regulated kinase (ERK) is a member of the mitogen-activated protein kinase family that is involved in both vasoconstriction and vascular smooth muscle cell growth and …
We begin with a brief overview of the structure, regulation, substrate recognition and subcellular localization of ERK1 and ERK2. We then systematically discuss how ERK signalling regulates …
Aug 10, 2017 · In this study, we describe and characterize novel sensors based on the Systematic Protein Affinity Strength Modulation (SPASM) technique. Sensors consist of a GPCR and G …
Dec 12, 2011 · Our studies demonstrate the use of the ER/K α-helix to systematically engineer FRET biosensors that detect changes in concentration or affinity of interacting proteins, and …
