• Source: 1,4-lactonase

The enzyme 1,4-lactonase (EC 3.1.1.25) catalyzes the generic reaction

a 1,4-lactone + H2O



⇌


{\displaystyle \rightleftharpoons }

a 4-hydroxyacid
This enzyme belongs to the family of hydrolases, specifically those acting on carboxylic ester bonds. The systematic name is 1,4-lactone hydroxyacylhydrolase. It is also called γ-lactonase. It participates in galactose metabolism and ascorbate and aldarate metabolism. It employs one cofactor, Ca2+.




Structural studies


As of late 2007, three structures have been solved for this class of enzymes, with PDB accession codes 2DG0, 2DG1, and 2DSO.




Applications


In a study by Chen et al. a 1,4-lactonase was expressed in E. coli and used as a highly efficient biocatalyst for asymmetric synthesis of chiral compounds.




References



Fishbein WN, Bessman SP (1966). "Purification and properties of an enzyme in human blood and rat liver microsomes catalyzing the formation and hydrolysis of γ-lactones. I. Tissue localization, stoichiometry, specificity, distinction from esterase". J. Biol. Chem. 241 (21): 4835–41. doi:10.1016/S0021-9258(18)99641-9. PMID 4958984.
Fishbein WN, Bessman SP (1966). "Purification and properties of an enzyme in human blood and rat liver microsomes catalyzing the formation and hydrolysis of γ-lactones. II. Metal ion effects, kinetics, and equilibra". J. Biol. Chem. 241 (21): 4842–7. doi:10.1016/S0021-9258(18)99642-0. PMID 4958985.

Kata Kunci Pencarian:

• 1,4-lactonase
• Lactonase
• Xylono-1,4-lactonase
• L-rhamnono-1,4-lactonase
• Γ-Butyrolactone
• Carboxymethylenebutenolidase
• Lactone
• List of EC numbers (EC 3)