- Source: 1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate dehydrogenase
In enzymology, a 1,6" target="_blank">1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate dehydrogenase (EC 1.3.1.25) is an enzyme that catalyzes the chemical reaction
(1R,6R)-1,6" target="_blank">1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD+
⇌
{\displaystyle \rightleftharpoons }
catechol + CO2 + NADH + H+
Thus, the two substrates of this enzyme are (1R,6R)-1,6" target="_blank">1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate and NAD+, whereas its 4 products are catechol, CO2, NADH, and H+.
This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-CH group of donor with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is (1R,6R)-1,6" target="_blank">1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate:NAD+ oxidoreductase (decarboxylating). Other names in common use include 3,5-cyclohexadiene-1,2-diol-1-carboxylate dehydrogenase, 3,5-cyclohexadiene-1,2-diol-1-carboxylic acid dehydrogenase, dihydrodihydroxybenzoate dehydrogenase, DHBDH, cis-1,2-dihydroxycyclohexa-3,5-diene-1-carboxylate dehydrogenase, 2-hydro-1,2-dihydroxybenzoate dehydrogenase, cis-1,2-dihydroxycyclohexa-3,5-diene-1-carboxylate:NAD+, oxidoreductase, and dihydrodihydroxybenzoate dehydrogenase. This enzyme participates in benzoate degradation via hydroxylation and benzoate degradation via coa ligation.
References
Reiner AM (1972). "Metabolism of aromatic compounds in bacteria. Purification and properties of the catechol-forming enzyme, 3,5-cyclohexadiene-1,2-diol-1-carboxylic acid (NAD + ) oxidoreductase (decarboxylating)". J. Biol. Chem. 247 (16): 4960–5. PMID 4341530.
Neidle E, Hartnett C, Ornston LN, Bairoch A, Rekik M, Harayama S (1992). "cis-diol dehydrogenases encoded by the TOL pWW0 plasmid xylL gene and the Acinetobacter calcoaceticus chromosomal benD gene are members of the short-chain alcohol dehydrogenase superfamily". Eur. J. Biochem. 204 (1): 113–20. doi:10.1111/j.1432-1033.1992.tb16612.x. PMID 1740120.
