- Source: 1965 Calgary Stampeders season
The 1965 Calgary Stampeders finished in first place in the Western Conference of the Canadian Football League with a 12–4 record. They were defeated in the Western Finals by the Winnipeg Blue Bombers.
Regular season
= Season standings
== Season schedule
=Playoffs
= Conference finals
=Winnipeg wins the best of three series 2–1. The Blue Bombers will advance to the Grey Cup Championship game.