- Source: 2021 Quito Challenger
The 2021 Quito Challenger was a professional tennis tournament played on clay courts. It was the 22nd edition of the tournament which was part of the 2021 ATP Challenger Tour. It took place in Quito, Ecuador between 13 and 19 September 2021.
Singles main-draw entrants
= Seeds
=1 Rankings are as of 30 August 2021.
= Other entrants
=The following players received wildcards into the singles main draw:
Álvaro Guillén Meza
Antonio Cayetano March
Gian Carlos Rodríguez
The following players received entry from the qualifying draw:
Luca Castelnuovo
Alexis Gautier
Alejandro Gómez
Christian Langmo
Champions
= Singles
=Facundo Mena def. Gonzalo Lama 6–4, 6–4.
= Doubles
=Alejandro Gómez / Thiago Agustín Tirante def. Adrián Menéndez Maceiras / Mario Vilella Martínez 7–5, 6–7(5–7), [10–8].