- Source: 4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase
In enzymology, a 3/info/4" target="_blank">4-3/info/galactosyl" target="_blank">galactosyl-3/info/n" target="_blank">N-3/info/acetylglucosaminide" target="_blank">acetylglucosaminide 3-alpha-L-fucosyltransferase (EC 2.3/info/4" target="_blank">4.1.152) is an enzyme that catalyzes the chemical reaction
GDP-beta-L-fucose + 1,3/info/4" target="_blank">4-beta-D-3/info/galactosyl" target="_blank">galactosyl-3/info/n" target="_blank">N-acetyl-D-glucosaminyl-R
⇌
{\displaystyle \rightleftharpoons }
GDP + 1,3/info/4" target="_blank">4-beta-D-3/info/galactosyl" target="_blank">galactosyl-(alpha-1,3-L-fucosyl)-3/info/n" target="_blank">N-acetyl-D-glucosaminyl- R
Thus, the two substrates of this enzyme are GDP-beta-L-fucose and 1,3/info/4" target="_blank">4-beta-D-3/info/galactosyl" target="_blank">galactosyl-3/info/n" target="_blank">N-acetyl-D-glucosaminyl-R. Its 3 products are GDP, 1,3/info/4" target="_blank">4-beta-D-3/info/galactosyl" target="_blank">galactosyl-(alpha-1,3-L-fucosyl)-3/info/n" target="_blank">N-acetyl-D-glucosaminyl-, and R.
This enzyme belongs to the family of glycosyltransferases, specifically the hexosyltransferases. The systematic name of this enzyme class is GDP-beta-L-fucose:1,3/info/4" target="_blank">4-beta-D-3/info/galactosyl" target="_blank">galactosyl-3/info/n" target="_blank">N-acetyl-D-glucosaminyl-R 3-alpha-L-fucosyltransferase. Other names in common use include:
Lewis-negative alpha-3-fucosyltransferase
plasma alpha-3-fucosyltransferase
guanosine diphosphofucose-glucoside alpha1→3-fucosyltransferase
galactoside 3-fucosyltransferase
GDP-L-fucose:1,3/info/4" target="_blank">4-beta-D-3/info/galactosyl" target="_blank">galactosyl-3/info/n" target="_blank">N-acetyl-D-glucosaminyl-R
3-L-fucosyltransferase
GDP-beta-L-fucose:1,3/info/4" target="_blank">4-beta-D-3/info/galactosyl" target="_blank">galactosyl-3/info/n" target="_blank">N-acetyl-D-glucosaminyl-R, and
3-L-fucosyltransferase
This enzyme participates in 3 metabolic pathways: glycosphingolipid biosynthesis - neo-lactoseries, glycosphingolipid biosynthesis - globoseries, and glycan structures - biosynthesis 2.
Structural studies
As of late 2007, 3 structures have been solved for this class of enzymes, with PDB accession codes 2NZW, 2NZX, and 2NZY.
References
Johnson PH, Yates AD, Watkins WM (1981). "Human salivary fucosyltransferases : evidence for two distinct alpha-3-L-fucosyltransferase activities one or which is associated with the Lewis blood group Le gene". Biochem. Biophys. Res. Commun. 100 (3/info/4" target="_blank">4): 1611–8. doi:10.1016/0006-291X(81)90703-8. PMID 7295318.
Schachter H, Narasimhan S, Gleeson P, Vella G (1983). "Glycosyltransferases involved in elongation of 3/info/n" target="_blank">N-glycosidically linked oligosaccharides of the complex or 3/info/n" target="_blank">N-acetyllactosamine type". Biomembranes Part L: Membrane Biogenesis: Processing and Recycling. Methods in Enzymology. Vol. 98. pp. 98–134. doi:10.1016/0076-6879(83)98143-0. ISBN 978-0-12-181998-9. PMID 6366476.
Ma B, Wang G, Palcic MM, Hazes B, Taylor DE (2003). "C-terminal amino acids of Helicobacter pylori alpha1,3/3/info/4" target="_blank">4 fucosyltransferases determine type I and type II transfer". J. Biol. Chem. 278 (24): 21893–900. doi:10.1074/jbc.M301704200. PMID 12676935.