- Source: 4-oxoproline reductase
In enzymology, a 4-oxoproline reductase (EC 1.1.1.104) is an enzyme that catalyzes the chemical reaction
4-oxo-L-proline + NADH + H+
⇌
{\displaystyle \rightleftharpoons }
cis-4-hydroxy-L-proline + NAD+
Thus, the three substrates of this enzyme are 4-oxo-L-proline, NADH, and H+, whereas its two products are cis-4-hydroxy-L-proline and NAD+.
This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is 4-hydroxy-L-proline:NAD+ oxidoreductase. This enzyme is also called hydroxy-L-proline oxidase. This enzyme was originally thought to participate in the metabolism of arginine and proline. However, recent data show that it is unlikely since neither 4-oxo-L-proline nor cis-4-hydroxy-L-proline are metabolites of these metabolic pathways.
Gene
The gene encoding 4-oxo-L-proline reductase was identified as 3-hydroxybutyrate dehydrogenase 2 (BDH2) by Sebastian Kwiatkowski and co-workers in 2022. The enzyme is a member of the Short-chain Dehydrogenases/Reductases (SDR) family of enzymes.
