• Source: Bicinchoninic acid

Bicinchoninic acid () or BCA is a weak acid composed of two carboxylated quinoline rings. It is an organic compound with the formula (C9H5NCO2H)2. The molecule consists of a pair of quinoline rings, each bearing a carboxylic acid group. Its sodium salt forms a purple complex with cuprous ions.
Bicinchoninic acid is most commonly employed in the bicinchoninic acid (BCA) assay, which is used to determine the total concentration of protein in a solution. Bicinchoninic acid is used to detect the presence of cuprous ions, due to its purple coloration via a biuret reaction. In this assay, two molecules of bicinchoninic acid chelate a single Cu+ ion, forming a purple water-soluble complex that strongly absorbs light at 562 nm.




References

Kata Kunci Pencarian:

• Bicinchoninic acid assay
• Bicinchoninic acid
• Biuret test
• Virus quantification
• BCA
• Dumas method
• Kjeldahl method
• Assay
• Protein purification
• Colorimetry (chemical method)