• Source: Calf-intestinal alkaline phosphatase

Calf-intestinal alkaline phosphatase (CIAP/CIP) is a type of alkaline phosphatase that catalyzes the removal of phosphate groups from the 5' end of DNA strands and phosphomonoesters from RNA. This enzyme is frequently used in DNA sub-cloning, as DNA fragments that lack the 5' phosphate groups cannot ligate. This prevents recircularization of the linearized DNA vector and improves the yield of the vector containing the appropriate insert.




Applications


Calf-intestinal alkaline phosphatase can serve as an effective tool for removing uranium from groundwater and soil that can pose major health risks. Furthermore, the toxicity of lipopolysaccharide (LPS) was mitigated by calf-intestinal alkaline phosphatase in mice and piglets, which indicates that it could be a promising new therapeutic agent for treating diseases associated with LPS.




References

Kata Kunci Pencarian:

• Calf-intestinal alkaline phosphatase
• Alkaline phosphatase
• Calf (animal)
• Gastrointestinal tract
• CIP
• Subcloning
• Ligation (molecular biology)
• Phytase
• Paucimannosylation