- Source: Coenzyme B
Coenzyme B is a coenzyme required for redox reactions in methanogens. The full chemical name of coenzyme B is 7-mercaptoheptanoylthreoninephosphate. The molecule contains a thiol, which is its principal site of reaction.
Coenzyme B reacts with 2-methylthioethanesulfonate (methyl-Coenzyme M, abbreviated CH3–S–CoM), to release methane in methanogenesis:
CH3–S–CoM + HS–CoB → CH4 + CoB–S–S–CoM
This conversion is catalyzed by the enzyme methyl coenzyme M reductase, which contains cofactor F430 as the prosthetic group.
A related conversion that utilizes both HS-CoB and HS-CoM is the reduction of fumarate to succinate, catalyzed by fumarate reductase:
HS–CoM + HS–CoB + −O2CCH=CHCO−2 → −O2CCH2–CH2CO−2 + CoB–S–S–CoM
Importance of coenzyme B in methanogenesis
Coenzyme B is an important component in the terminal step of methane biogenesis. It acts as a two electron-donor to reduce coenzyme M (methyl-coenzyme) into two molecules a methane and a heterodisulfide. Two separate experiments that were performed, one with coenzyme B and other without coenzyme B, indicated that using coenzyme B before the formation of the methane molecule, results in a more efficient and consistent bond cleavage.