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Ent-kaurenoic acid oxidase (EC 1.14.13.79) is an enzyme with systematic name ent-kaur-16-en-19-oate,NADPH:oxygen oxidoreductase (hydroxylating). This enzyme catalyses the following chemical reaction
ent-kaur-16-en-19-oate + 3 NADPH + 3 H+ + 3 O2
⇌
{\displaystyle \rightleftharpoons }
gibberellin A12 + 3 NADP+ + 4 H2O (overall reaction)
(1a) ent-kaur-16-en-19-oate + NADPH + H+ + O2
⇌
{\displaystyle \rightleftharpoons }
ent-7alpha-hydroxykaur-16-en-19-oate + NADP+ + H2O
(1b) ent-7alpha-hydroxykaur-16-en-19-oate + NADPH + H+ + O2
⇌
{\displaystyle \rightleftharpoons }
gibberellin A12 aldehyde + NADP+ + 2 H2O
(1c) gibberellin A12 aldehyde + NADPH + H+ + O2
⇌
{\displaystyle \rightleftharpoons }
gibberellin A12 + NADP+ + H2O
Ent-kaurenoic acid oxidase requires cytochrome P450.
