- Source: Feruloyl esterase
The enzyme feruloyl esterase (EC 3.1.1.73) catalyzes the reaction
feruloyl-polysaccharide + H2O
⇌
{\displaystyle \rightleftharpoons }
ferulate + polysaccharide
This enzyme belongs to the family of hydrolases, specifically those acting on carboxylic ester bonds. The systematic name is 4-hydroxy-3-methoxycinnamoyl-sugar hydrolase. Other names in common use include ferulic acid esterase (FAE), hydroxycinnamoyl esterase, hemicellulase accessory enzyme, cinnamoyl ester hydrolase (cinnAE).
Structural studies
As of late 2007, 6 structures have been solved for this class of enzymes, with PDB accession codes 1USW, 1UWC, 1UZA, 2BJH, 2HL6, and 2IX9.
References
Catherine Tomaro-Duchesneau; Shyamali Saha; Meenakshi Malhotra; Michael Coussa-Charley; Imen Kahouli; Mitchell L. Jones; Alain Labbe; Satya Prakash (2012). "Probiotic Ferulic Acid Esterase Active Lactobacillus fermentum NCIMB 5221 APA Microcapsules for Oral Delivery: Preparation and in Vitro Characterization". Pharmaceuticals. 5 (2): 236–248. doi:10.3390/ph5020236. PMC 3763630. PMID 24288090.
D.B.R.K. Gupta Udatha; Irene Kouskoumvekaki; Lisbeth Olsson; Gianni Panagiotou (2011). "The interplay of descriptor-based computational analysis with pharmacophore modeling builds the basis for a novel classification scheme for feruloyl esterases". Biotechnology Advances. 29 (1): 94–110. doi:10.1016/j.biotechadv.2010.09.003. PMID 20851174.
Faulds CB, Williamson G (1991). "The purification and characterization of 4-hydroxy-3-methoxycinnamic (ferulic) acid esterase from Streptomyces olivochromogenes". J. Gen. Microbiol. 137 (10): 2339–45. doi:10.1099/00221287-137-10-2339. PMID 1663152.
Faulds CB, Williamson G (1994). "Purification and characterisation of a ferulic acid esterase (FAE-III) from Aspergillus niger. Specificity for the phenolic moiety and binding to microcrystalline cellulose". Microbiology. 140 (4): 779–787. doi:10.1099/00221287-140-4-779.
Kroon PA, Faulds CB, Williamson G (June 1996). "Purification and characterization of a novel esterase induced by growth of Aspergillus niger on sugar-beet pulp". Biotechnol. Appl. Biochem. 23 (3): 255–62. doi:10.1111/j.1470-8744.1996.tb00381.x. PMID 8679110. S2CID 19514727.
Faulds CB, Williamson G, van den Hombergh JP, Visser J (1997). "The faeA genes from Aspergillus niger and Aspergillus tubingensis encode ferulic acid esterases involved in degradation of complex cell wall polysaccharides". Appl. Environ. Microbiol. 63 (12): 4638–44. Bibcode:1997ApEnM..63.4638D. doi:10.1128/AEM.63.12.4638-4644.1997. PMC 168785. PMID 9406381.
Castanares A, Mccrae SI, Wood TM (1992). "Purification and properties of a feruloyl/p-coumaroyl esterase from the fungus Penicillium pinophilum". Enzyme and Microbial Technology. 14 (11): 875–884. doi:10.1016/0141-0229(92)90050-X.
