- Source: HA-tag
The HA-tag is a protein tag derived from the human influenza hemagglutinin (HA) protein, which allows the virus to target and enter host cells. An HA-tag is composed of a peptide derived from the HA-molecule corresponding to amino acids 98-106, which can be recognized and selectively bound by commercially available antibodies. This makes HA a powerful tool in molecular biology, commonly included in expression vectors and in the production of recombinant proteins.
Like other epitope tags, HA-tag is small and generally does not alter the traits of proteins it is attached to. As a result HA-tags are often used to identify protein-protein interactions or to detect protein expression, using Co-Immunoprecipitation or Western blot respectively.
The HA-tag is not suitable for detection or purification of proteins from apoptotic cells since it is cleaved by Caspase-3 and / or Caspase-7 after its sequence DVPD, causing it to lose its immunoreactivity. Labeling of endogenous proteins with HA-tag using CRISPR was recently accomplished in-vivo in differentiated neurons.
Sequence
The DNA sequences for the HA-tag include: 5'-TAC-CCA-TAC-GAT-GTT-CCA-GAT-TAC-GCT-3' or 5'-TAT-CCA-TAT-GAT-GTT-CCA-GAT-TAT-GCT-3'. The resulting amino acid sequence is YPYDVPDYA (Tyr-Pro-Tyr-Asp-Val-Pro-Asp-Tyr-Ala).
See also
Protein tag
SpyTag
References
Further reading
Kata Kunci Pencarian:
- Ha Jung-woo
- Choi Hyun-wook
- Saint-Louis-du-Ha! Ha!
- Frances Ha
- Aha ha
- Manuskrip Al-Qur'an Birmingham
- Kekuatan asam
- Kim Tae-ha
- Surströmming
- Nam Joo-hyuk
- HA-tag
- FLAG-tag
- Protein tag
- Skin tag
- Epitope
- Tandem affinity purification
- SBP-tag
- Targum Onkelos
- Christine Hà
- P24 capsid protein
John Wick: Chapter 3 – Parabellum (2019)
Crank: High Voltage (2009)
Hell House LLC Origins: The Carmichael Manor (2023)
36
Tomorrow Never Dies (1997)
No More Posts Available.
No more pages to load.
