- Source: INT (chemical)
INT (iodonitrotetrazolium or 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium) is a commonly used tetrazolium salt (usually prepared with chloride ions), similar to tetrazolium chloride that on reduction produces a red formazan dye that can be used for quantitative redox assays. It is also toxic to prokaryotes.
INT is an artificial electron acceptor that can be utilized in a colorimetric assay to determine the concentration of protein in a solution. It can be reduced by succinate dehydrogenase to furazan, the formation of which can be measured by absorbance at 490 nm. The activity of succinate dehydrogenase is readily observed by the naked eye as the solution turns from colorless to rusty red.
See also
MTT assay
References
Kata Kunci Pencarian:
- Energi kinetik
- Sukrosa
- Angewandte Chemie
- Amonium nitrat
- Tabel periodik
- Bilangan oksidasi
- Energi mekanis
- Bilangan iodin
- N-Butanol
- Molekul
- INT
- INT (chemical)
- Chemical potential
- Per- and polyfluoroalkyl substances
- Beer–Lambert law
- List of organizations with .int domain names
- List of chemical compounds with unusual names
- Molecule
- Potential energy
- Periodic table
