- Source: Lacto-N-biosidase
Lacto-N-biosidase (EC 3.2.1.140) is an enzyme with systematic name oligosaccharide lacto-N-biosylhydrolase. This enzyme catalyses the following chemical reaction
(1) beta-D-Gal-(1->3)-beta-D-GlcNAc-(1->3)-beta-D-Gal-(1->4)-D-Glc + H2O
⇌
{\displaystyle \rightleftharpoons }
beta-D-Gal-(1->3)-D-GlcNAc + beta-D-Gal-(1->4)-D-Glc
(2) lacto-N-tetraose + H2O
⇌
{\displaystyle \rightleftharpoons }
lacto-N-biose + lactose
The enzyme from Streptomyces specifically hydrolyses the terminal lacto-N-biosyl residue (beta-D-Gal-(1->3)-D-GlcNAc) from the non-reducing end of oligosaccharides with the structure beta-D-Gal-(1->3)-beta-D-GlcNAc-(1->3)-beta-D-Gal-(1->R).
References
External links
Lacto-N-biosidase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
