- Source: Multisite-specific tRNA:(cytosine-C5)-methyltransferase
Multisite-specific tRNA:(cytosine-C5)-methyltransferase (EC 2.1.1.202, multisite-specific tRNA:m5C-methyltransferase, TRM4 (gene)) is an enzyme with systematic name S-adenosyl-L-methionine:tRNA (cytosine-C5)-methyltransferase. This enzyme catalyses the following chemical reaction
(1) S-adenosyl-L-methionine + cytosine34 in tRNA precursor
⇌
{\displaystyle \rightleftharpoons }
S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNA precursor
(2) S-adenosyl-L-methionine + cytosine40 in tRNA precursor
⇌
{\displaystyle \rightleftharpoons }
S-adenosyl-L-homocysteine + 5-methylcytosine40 in tRNA precursor
(3) S-adenosyl-L-methionine + cytosine48 in tRNA
⇌
{\displaystyle \rightleftharpoons }
S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNA
(4) S-adenosyl-L-methionine + cytosine49 in tRNA
⇌
{\displaystyle \rightleftharpoons }
S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNA
The enzyme from Saccharomyces cerevisiae is responsible for complete 5-methylcytosine methylations of yeast tRNA.
References
External links
Multisite-specific+tRNA:(cytosine-C5)-methyltransferase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)