• Source: Nicking enzyme

A nicking enzyme (or nicking endonuclease) is an enzyme that cuts one strand of a double-stranded DNA or RNA at a specific recognition nucleotide sequences known as a restriction site. Such enzymes hydrolyze (cut) only one strand of the DNA duplex, to produce DNA molecules that are “nicked”, rather than cleaved.
They can be used for strand-displacement amplification, Nicking Enzyme Amplification Reaction, exonucleolytic degradation, the creation of small gaps, or nick translation. The latter process has been successfully used to incorporate both radioactively labelled nucleotides and fluorescent nucleotides allowing specific regions on a double stranded DNA to be studied. Over 200 nicking enzymes have been studied, and 13 of these are available commercially and are routinely used for research and in commercial products.




References






External links


New England Biolabs Nicking Enzymes
Fermentas Nicking Enzymes

Kata Kunci Pencarian:

• Nicking enzyme
• Nicking enzyme amplification reaction
• Near
• Nick (DNA)
• Topoisomerase
• RecBCD
• Variants of PCR
• Glossary of cellular and molecular biology (M–Z)
• Ionian Technologies
• DNA nanotechnology