- Source: P35 holin family
The PRD1 Phage P35 Holin (P35 Holin) Family (TC# 1.E.5) is a member of Holin Superfamily III. The prototype for this family is the lipid-containing PRD1 enterobacterial phage holin protein P35 (12.8 kDa; TC# 1.E.5.1.1) encoded by gene XXXV (orfT). It is a component of a typical holin-endolysin system which functions to lyse the host bacterial cell.
Structure
P35 holin (TC# 1.E.5.1.1) has 3 transmembrane segments (TMSs) with 5 positively charged residues between TMSs 1 and 2. It has 4 positively charged residues at the C-terminus. It is therefore thought that the N-terminus is in the periplasm and the C-terminus is in the cytoplasm. Homologues of 109 amino acyl residues (aas), which also have 3 putative TMSs, are encoded in the genomes of Xylella fastidiosa strains.
Function
The reaction catalyzed by P35 holin is:
autolysin (in) → autolysin (out)
See also
Bacteriophage
Phage typing
Holin
Lysin
Transporter Classification Database
References
As of 10 March 2016, this article is derived in whole or in part from Transporter Classification Database (TCDB). The copyright holder has licensed the content in a manner that permits reuse under CC BY-SA 3.0 and GFDL. All relevant terms must be followed. The original text was at "1.E.5 The PRD1 Phage P35 Holin (P35 Holin) Family"
