• Source: RhoC

RhoC (Ras homolog gene family, member C) is a small (~21 kDa) signaling G protein (more specifically a GTPase), and is a member of the Rac subfamily of the family Rho family of GTPases. It is encoded by the gene RHOC.




Mechanism and function


It is prenylated at its C-terminus, and localizes to the cytoplasm and plasma membrane. It is thought to be important in cell locomotion. It cycles between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades.
Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape and motility. RhoC can activate formins such as mDia1 and FMNL2 to remodel the cytoskeleton.
Overexpression of RhoC is associated with cell proliferation and causing tumors to become malignant. It causes degradation and reconstruction of the Extracellular Matrix (ECM) which helps cells escape the tissue they are currently in. It enhances cell motility giving it the ability to become invasive. It has been found to have a direct relationship to advanced tumor stage and metastasis, with increases in stage being related to increases in RhoC expression. RhoC-deficient mice can still develop tumors but these fail to metastasize, arguing that RhoC is essential for metastasis.
It has also been found to enhance the creation of angiogenic factors such as VEGF, which is necessary for a tumor to become malignant.
In a study by Vega, RhoC was knocked out which resulted in cells spreading out wide in all directions. When RhoC was disabled, the cell's abilities to move in a specific direction and migrate was impaired. It also reduced the cell's speed of movement, because it was difficult, and sometimes impossible, to polarize the cell.




Associated Signaling Pathways


RhoC expression has been associated with several signaling pathways and effectors. Here is a list of the ones found so far:

IQGAP1 (IQ-domain GTP-ase Activating Protein): an effector of RhoC to enhance expression of cyclin E and cyclin D1. This resulted in cells being promoted to enter S phase more rapidly
ROCK-1
MMP9: necessary for ECM regulation
FMNL3: a Formin downstream target, which is used to regulate where Rac1 is active
MAPK pathway: upregulating VEGF, Basic fibroblastic growth factors, and interleukins 6 and 8 expression
Notch1
PI3K/AKt pathway: Proliferation and invasiveness
Pyk2: metastasis




Types of Cancer RhoC has been studied in


RhoC has been found to be overexpressed in:

Lung Cancer
Gastric Cancer
Ovarian cancer
Breast Cancer
Hepatocellular Cancer
Pancreatic Cancer
Colorectal Cancer
Cancer of the Urogenital System
Melanoma
Prostate Cancer
Cervical Carcinoma




Potential Therapies


RhoC small interfering RNA (siRNA) have been used in studies to successfully inhibit proliferation of some invasive cancers
RhoC can be used as a biomarker for judging the metastatic potential of tumors
One study used "recombinant adenovirus mediated RhoC shRNA in tandem linked expression" to successfully inhibit RhoC
It has been found that RhoC expression is not important for embryogenesis but it is only important for metastasis, which would make it a good target for treatments.
A RhoC targeted therapy (RV001 by RhoVac) is currently tested in prostate cancer in an ongoing clinical phase 2b program in the US and Europe. Results are expected mid 2022 (Reference: https://clinicaltrials.gov/ct2/show/NCT04114825)




References






Further reading






External links


Laudanna C (2008). "RhoC". AfCS-Nature Molecule Pages. doi:10.1038/mp.a002065.01 (inactive 1 November 2024).{{cite journal}}: CS1 maint: DOI inactive as of November 2024 (link)
"RhoC : Abstract : UCSD-Nature Molecule Pages". Archived from the original on 2021-12-04. Retrieved 2009-06-16.

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