- Source: Route 16
- Nevada State Route 160
- Jalan Raya Nasional Jepang Rute 16
- U.S. Route 2
- U.S. Route 3
- U.S. Route 1
- U.S. Route 24
- Coles County, Illinois
- Christian County, Illinois
- Edgar County, Illinois
- Calhoun County, Illinois
- Route 16
- U.S. Route 16
- California State Route 16
- U.S. Route 60
- Massachusetts Route 16
- New Hampshire Route 16
- Washington State Route 16
- Melbourne tram route 16
- U.S. Route 16 in Michigan
- Route-16 (video game)
Route 16 may refer to:
One of several highways - see List of highways numbered 16
One of several public transport routes - see List of public transport routes numbered 16
Mystic Valley Parkway station, also called Route 16 - a proposed light rail station in Medford, Massachusetts
Route-16 (video game), a 1981 video game