- Source: RTCB
RNA 2',3'-cyclic phosphate and 5'-OH ligase is a protein that in humans is encoded by the RTCB gene. It is found in the stress granule of cells.
Structure
As of June 2019, no crystal structure of the human RTCB is known, but homology models built from other RtcB-family ligases are available (Swiss-model: Q9Y3I0). The structure of Pyrococcus horikoshii RtcB, which uses GTP instead of ATP, shows two manganese (Mn2+) cofactors, and a mechanism involving a covalently linked GTP-histidine-RtcB intermediate. The residue involved, H404, is conserved in human RTCB as H428.
Function
Protein family
RTCB belongs to the RtcB family of ATP-dependent RNA ligases, named after the eponymous protein in E. coli. The bacterial RtcB acts as a tRNA ligase, rejoining broken stem-loops in case of damage. It is also able to catalyse RNA splicing.
The eukaryotic homologs of RtcB, including the human RTCB protein, participates in the tRNA-splicing ligase complex.
References
Further reading
External links
MetaCyc: CPLX-9136: Homo sapiens tRNA-splicing ligase complex (GO:0072669)
