- Source: Sulforhodamine B
Sulforhodamine B or Kiton Red 620 (C27H30N2O7S2) is a fluorescent dye with uses spanning from laser-induced fluorescence (LIF) to the quantification of cellular proteins of cultured cells. This red solid dye is very water-soluble.
Spectroscopy
The dye has maximal absorbance at 565 nm light and maximal fluorescence emission at 586 nm light. It does not exhibit pH-dependent absorption or fluorescence over the range of 3 to 10.
Applications
Sulforhodamine B is often used as a membrane-impermeable polar tracer or used for cell density determination via determination of cellular proteins (cytotoxicity assay).
References
Kata Kunci Pencarian:
- Asam sulfonat
- Sulforhodamine B
- Cytotoxicity
- Assay
- Pannexin
- Dye tracing
- DIRAS1
- E-SCREEN
- Rhodamine
- Cyanine
- List of dyes