- Source: TRNA dimethylallyltransferase
TRNA dimethylallyltransferase (EC 2.5.1.75, tRNA prenyltransferase, MiaA, transfer ribonucleate isopentenyltransferase, Delta2-isopentenyl pyrophosphate:tRNA-Delta2-isopentenyl transferase, Delta2-isopentenyl pyrophosphate:transfer ribonucleic acid Delta2-isopentenyltransferase) is an enzyme with systematic name dimethylallyl-diphosphate: tRNA dimethylallyltransferase. This enzyme catalyses the following chemical reaction
dimethylallyl diphosphate + tRNA
⇌
{\displaystyle \rightleftharpoons }
diphosphate + tRNA containing 6-dimethylallyladenosine
Formerly known as tRNA isopentenyltransferase (EC 2.5.1.8), but it is now known that dimethylallyl diphosphate, rather than isopentenyl diphosphate, is the substrate.
Structural studies
As of late 2007, only one structure has been solved for this class of enzymes, with the PDB accession code 2QGN.
References
Literature
Kline LK, Fittler F, Hall RH (1969). "N6-(Δ2-isopentenyl)adenosine. Biosynthesis in transfer ribonucleic acid in vitro". Biochemistry. 8 (11): 4361–4371. doi:10.1021/bi00839a021. PMID 4311031.
Rosenbaum N, Gefter ML (1972). "Δ2-Isopentenylpyrophosphate: Transfer Ribonucleic Acid Δ2-Isopentenyltransferase from Escherichia coli. Purification and properties of the enzyme". J. Biol. Chem. 247 (18): 5675–5680. doi:10.1016/S0021-9258(19)44812-6. PMID 4341485.
External links
TRNA+dimethylallyltransferase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)