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    CRISPR (Bahasa Inggris: Clustered regularly interspaced short palindromic repeats, dilafalkan crisper) adalah bagian dari DNA prokariota yang mengandung urutan dasar pendek, berulang. Dalam suatu pengulangan palindrom, urutan nukleotida adalah sama dalam kedua arah. Setiap pengulangan diikuti oleh bagian pendek DNA pengatur jarak dari paparan sebelumnya oleh DNA asing (yakni suatu virus atau plasmid). Kelompok kecil dari gen cas (CRISPR-associated system) terletak di samping urutan CRISPR.
    Sistem CRISPR/Cas adalah suatu sistem kekebalan prokariota yang memberikan perlawanan terhadap elemen genetik asing seperti yang hadir dalam plasmid dan bakteriofag yang menyediakan bentuk sistem imun adaptif. RNA menyimpan urutan pengatur jarak yang membantu protein Cas mengenali dan memotong DNA eksogen. Protein Cas yang dipandu RNA lainnya memotong RNA asing. CRISPR ditemukan dalam sekitar 40% dari genom bakteri yang telah disekuensing dan 90% arkea yang telah disekuensing.
    Versi sederhana CRISPR/Cas yang disebut CRISPR/Cas9 telah digunakan untuk memodifikasi genom. Genom sel dapat dipotong ke tempat yang diinginkan, sehingga gen yang ada dapat dihilangkan dan yang baru ditambahkan. Teknik ini dapat digunakan untuk berbagai macam hal, dari kedokteran hingga peningkatan mutu bibit.


    Lihat juga


    Anti-CRISPR
    Interferensi RNA
    Biologi sintetis


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    Referensi




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    Jennifer Doudna talk: Genome Engineering with CRISPR-Cas9: Birth of a Breakthrough Technology

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CRISPR Cas9 Explained - YouTube

CRISPR Cas9 Explained - YouTube

CRISPR

CRISPR

CRISPR

CRISPR

CRISPR

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CRISPR screening - Innovative Genomics Institute (IGI)

CRISPR screening - Innovative Genomics Institute (IGI)

CRISPR – All about microbes!

CRISPR – All about microbes!

CRISPR for Clinicians – Medical Education Speakers Network

CRISPR for Clinicians – Medical Education Speakers Network

What is CRISPR? - FactsandHistory

What is CRISPR? - FactsandHistory

What is CRISPR? - MIT McGovern Institute

What is CRISPR? - MIT McGovern Institute

Keeping the genetic code clean

Keeping the genetic code clean

Whatever happened to CRISPR? - Harvard Health

Whatever happened to CRISPR? - Harvard Health

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What is CRISPR? A bioengineer explains | Stanford Report

Jun 10, 2024 · Learn how CRISPR works, why it's a big deal, and what it can do for medicine, biotechnology, and the environment. A Q&A with bioengineer Stanley Qi, who explains the …

CRISPR gene editing - Wikipedia

Learn about CRISPR, a genetic engineering technique based on a bacterial defense system that can modify genomes in vivo. Find out its history, …

CRISPR - National Human Genome Research Institute

3 days ago · CRISPR (short for “clustered regularly interspaced short palindromic repeats”) is a technology that research scientists use to selectively modify the DNA of living organisms. CRISPR was adapted for use in the laboratory from …

What is CRISPR? - New Scientist

CRISPR is a way of finding and altering a specific bit of DNA inside a cell, using a protein called Cas9 and a guide RNA. It can be used for scientific research, medicine, agriculture and more, but also raises ethical and environmental …

What Is CRISPR Gene Editing and How Does It Work?

Apr 25, 2023 · Learn how CRISPR works as a gene editing tool and what it can do for health conditions like sickle cell anemia and hemophilia. Find out the current status of CRISPR clinical trials and the ethical issues involved.

What is CRISPR/Cas9? - PMC - PubMed Central (PMC)

CRISPR/Cas9 is a gene-editing technology which involves two essential components: a guide RNA to match a desired target gene, and Cas9 (CRISPR-associated protein 9)—an endonuclease which causes a double-stranded …

Mechanism and Applications of CRISPR/Cas-9-Mediated …

Clustered regularly interspaced short palindromic repeat (CRISPR) and their associated protein (Cas-9) is the most effective, efficient, and accurate method of genome editing tool in all living …

CRISPR–Cas9: A History of Its Discovery and Ethical …

CRISPR – clustered regularly interspaced short palindromic repeats – were first discovered in the sequences of DNA from Escherichia coli bacteria and described in 1987 by Ishino et al. [1] from Osaka University (Japan).