Fluoro-Jade C Staining. Brain sections stained with Fluoro-Jade C ...
Fluoro Jade B staining question? | ResearchGate
Fluoro Jade B staining question? | ResearchGate
biosensis® Ready-to-Dilute (RTD) Fluoro-Jade C Staining Kit Protocol
Fluoro-Jade®C | AAT Bioquest
Hippocampal neurodegeneration (assessed by Fluoro-Jade C staining ...
Fluoro-Jade C staining to show early neuronal degeneration. A ...
Fluoro-Jade C staining to show early neuronal degeneration. A ...
Fluoro Jade C staining of medial hippocampus CA1 revealed neuronal ...
Immunohistochemistry study by staining with Fluoro-Jade C, NeuN, and ...
Comparison of Fluoro-Jade C (FJC) staining in the SNpc between: (A ...
Comparison of Fluoro-Jade C (FJC) staining in the SNpc between: (A ...
The fluorescent dye contained in the Fluoro-Jade® C (FJC) Ready-to-dilute (RTDTM) staining kit stains for neuronal degeneration and can be used with other fluorescent Nissl stains or UV nuclear stain such as DAPI (included) to help visualize the cells and cell nuclei. The reagents are all supplied in a liquid format and are ready-to-dilute.
Dissolve 10 mg of Fluoro-Jade® C powder in 100 mL distilled water, filter through a 0.45 μm membrane and store at 4°C in the dark for up to 3 months. Discard if cloudy or precipitated. Add 1 mL of the FJC 0.01% stock to 99 mL of solution of 0.1% acetic acid vehicle to make a 0.0001% working solution.
staining protocols. Triple labeling can be accomplished by staining degenerating neurons with Fluoro-Jade C, cell nuclei with DAPI and activated astrocytes with GFAP immunofluorescence.
fluoro-jade c histological staining protocol: Half of each group of brains were paraffin embedded and cut on a rotary microtome while the remainder were cut on a freezing sliding microtome. Paraffin sections were 10um in thickness while frozen sections were cut at a thickness of 25um.
Jul 12, 2023 · The dye is highly resistant to fading and is compatible with virtually all histological processing and staining protocols. Triple labeling can be accomplished by staining degenerating neurons with Fluoro-Jade C, cell nuclei with DAPI and activated astrocytes with GFAP immunofluorescence.
Detailed protocol for Fluoro-Jade C staining of brain slices to visualize and quantify damaged neurons around a hematoma.
Jan 15, 2020 · Fluoro-Jade C (FJC) staining is widely used for the specific detection of all degenerating mature neurons, including apoptotic, necrotic, and autophagic cells. However, whether FJC staining can detect degenerating immature neurons and neural stem/precursor cells remains unclear.
The biosensis® RTDTM (Ready-to-Dilute) Fluoro-Jade® C staining kit is designed to stain for neuronal degeneration with a fluorescent Nissl counter stain. The reagents are all supplied in a liquid format and are ready-to-dilute.
Fluoro-Jade® C stains all degenerating neurons regardless of specific insult or mechanism of cell death. Fluoro-Jade C exhibits the greatest signal to background ratio, as well as the highest resolution. This translates to a stain of maximal contrast and affinity for degenerating neurons.
Using our improved double staining protocol with FJC and FI, we detected FJC-labeled degenerating astrocytes and oligodendrocytes with pyknotic nuclei. These results indicate that FJC is not specific to degenerating neurons in some experimental conditions: Keywords: damaged cells, glia, histochemistry, improved protocol, injury.
