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The Neogen Petrifilm plate is an all-in-one plating system made by the Food Safety Division of the Neogen Corporation. They are heavily used in many microbiology-related industries and fields to culture various micro-organisms and are meant to be a more efficient method for detection and enumeration compared to conventional plating techniques. A majority of its use is for the testing of foodstuffs.
Petrifilm plates are designed to be as accurate as conventional plating methods. Ingredients usually vary from plate to plate depending on what micro-organism is being cultured, but generally a Petrifilm comprises a cold-water-soluble gelling agent, nutrients, and indicators for activity and enumeration.
A typical Petrifilm plate has a 10 cm(H) × 7.5 cm(W) bottom film which contains a foam barrier accommodating the plating surface, the plating surface itself (a circular area of about 20 cm2), and a top film which encloses the sample within the Petrifilm. A 1 cm × 1 cm yellow grid is printed on the back of the plate to assist enumeration. A plastic “spreader” is also used to spread the inoculum evenly.
Comparisons between Petrifilm plates and standard methods
Petrifilm plates have become widely used because of their cost-effectiveness, simplicity, convenience, and ease of use. For example, conventional plating would require preparing agar for pour plating, or using agar plates and vial inoculum loops for streak plating; but for Petrifilm plates, the agar is completely housed in a single unit so that only the sample has to be added, which saves time.
For incubation, Petrifilm plates can be safely stacked and incubated just like Petri dishes. Since they are paper thin, more plates can be stacked together than Petri dishes (although it is recommended that Petrifilms be stacked no higher than 20).
For enumeration, Petrifilm plates can be used on any colony counter for enumeration just like a Petri dish. Various enumeration experiments have shown very little or no variance between counts obtained through Petrifilm and standard agar counts. In some cases, Petrifilms were more sensitive in detection than standard microbiology methods, other than the case that a higher sensitivity could possibly lead to an increased risk of false positive results.
Procedure for using Petrifilm plates
First, a sample must be prepared through standard weighing and serial dilution, with stomaching and pH adjustment if necessary.
Next, to inoculate, the top layer is lifted to expose the plating surface, and with a pipette, 1mL of the diluted sample is added. The top film is then slowly rolled down and the “spreader” is used for even distribution. It takes a minute for gelling to occur.
After incubation
After the required incubation period, colonies appear either as splotches, spots which are surrounded by bubbles, or a combination of both, which differ from micro-organism to micro-organism. Enumeration can be done on a standard colony counter. Picking out individual colonies for interpretation can also be done because the top film can be lifted quite effortlessly to expose the gel. Unfortunately, if a sample is too dark in colour (e.g. chocolate or hot chocolate), enumeration becomes more difficult or impossible, since the stained colonies are less visible.
Types of Petrifilm plates
Aerobic plate count
Aerobic plate count with lactic acid bacteria
Lactic acid bacteria
Coliform
Rapid coliform
High-Sensitivity coliform
E. coli / coliform
Enterobacteriaceae
Staphylococcus express
Yeast and mould
Environmental listeria
References
External links
Petrifilm Plates
Neogen Petrifilm Catalog
See also
Agar plate
Agar
Petri dish
Neogen Corporation
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Petrifilm - Wikipedia
Petrifilm plates are designed to be as accurate as conventional plating methods. Ingredients usually vary from plate to plate depending on what micro-organism is being cultured, but …
Petrifilm® Plates | Plates for Quality Indicator Testing - Neogen
Petrifilm microbiology plates are ready to use and require no prep. Compared to traditional agar methods, Petrifilm Plates could reduce labor costs by 45%, increase technician efficiency by …
Petrifilm for Food Safety | 3M United States
3M™ Petrifilm™ Plate Reader Advanced Power Cord, 6557PC-EU, 1 Each, European Union
Petrifilm® Aerobic Count Plate - Neogen
The Neogen® Petrifilm® Aerobic Count (AC) Plate is a time-saving, sample-ready plate designed to determine total aerobic bacteria populations. A built-in grid facilitates counting colonies, …
Petrifilm® Enterobacteriaceae Count Plates - Neogen
The Neogen® Petrifilm® Enterobacteriaceae Count Plate provides results in just 24 hours, enabling you to quickly determine potential sources of contamination. Enterobacteriaceae …
3M™ Petrifilm™ Aerobic Count Plates 6406, 1000 each/case
Each 3M Petrifilm Aerobic Count Plate contains a water-soluble gelling agent, nutrients and indicator — all the components needed for microbial growth with no agar preparation required. …
3M™ Petrifilm™ Rapid Aerobic Count (RAC) Plate Method for the ...
Jun 16, 2021 · The 3M™ Petrifilm™ Rapid Aerobic Count (RAC) Plate is a sample-ready culture medium system designed to facilitate colony enumeration in 24 h (48 h for dry powders) for …
Petrifilm Microbiology Indicator Plates & Readers | 3M United States
Equipment used to monitor hygienic conditions by counting and documenting the presence of microbiology indicator organisms. Includes plates prepared with culture media that are used to …
3M™ Petrifilm™ Yeast and Mold Count Plate 6407, 100 per box
This 3M Petrifilm Yeast and Mold Count Plate is a sample-ready-culture medium system which contains nutrients supplmented with antibiotics, a cold-water-soluble gelling agent, and an …
Petrifilm™ Rapid Aerobic Count Plates, 500-pk
Petrifilm™ Rapid Aerobic Count Plates, 500-pk. Provides results within 24 hours of incubation time (except for dairy powders). Plates are sample-ready, eliminating all the time-consuming …