- Source: Glucosylceramidase
In enzymology, a glucosylceramidase (EC 3.2.1.45) is an enzyme that catalyzes the chemical reaction
D-glucosyl-N-acylsphingosine + H2O
⇌
{\displaystyle \rightleftharpoons }
D-glucose + N-acylsphingosine
Thus, the two substrates of this enzyme are D-glucosyl-N-acylsphingosine and H2O, whereas its two products are D-glucose and N-acylsphingosine.
This enzyme belongs to the family of hydrolases, specifically those glycosidases that hydrolyse O- and S-glycosyl compounds. The systematic name of this enzyme class is D-glucosyl-N-acylsphingosine glucohydrolase. Other names in common use include:
psychosine hydrolase,
glucosphingosine glucosylhydrolase,
GlcCer-beta-glucosidase,
beta-D-glucocerebrosidase,
glucosylcerebrosidase,
beta-glucosylceramidase,
ceramide glucosidase,
glucocerebrosidase,
glucosylsphingosine beta-glucosidase,
and glucosylsphingosine beta-D-glucosidase.
This enzyme participates in sphingolipid metabolism and degradation of glycan structures.
Human proteins containing this domain
GBA belongs to Glycoside hydrolase family 30, GBA2 belongs to Glycoside hydrolase family 116.
References
Further reading
Brady RO, Kanfer J, Shapiro D (1965). "The metabolism of glucocerebrosides: I. Purification and properties of a glucocerebroside-cleaving enzyme from spleen tissue". J. Biol. Chem. 240: 39–43. doi:10.1016/S0021-9258(18)97611-8. PMID 14253443.
Vaccaro AM, Muscillo M, Suzuki K (1985). "Characterization of human glucosylsphingosine glucosyl hydrolase and comparison with glucosylceramidase". Eur. J. Biochem. 146 (2): 315–21. doi:10.1111/j.1432-1033.1985.tb08655.x. PMID 3967661.