• Source: Nucleic acid methods

Nucleic acid methods are the techniques used to study nucleic acids: DNA and RNA.




Purification


DNA extraction
Phenol–chloroform extraction
Minicolumn purification
RNA extraction
Boom method
Synchronous coefficient of drag alteration (SCODA) DNA purification




Quantification


Abundance in weight: spectroscopic nucleic acid quantitation
Absolute abundance in number: real-time polymerase chain reaction (quantitative PCR)
High-throughput relative abundance: DNA microarray
High-throughput absolute abundance: serial analysis of gene expression (SAGE)
Size: gel electrophoresis




Synthesis


De novo: oligonucleotide synthesis
Amplification: polymerase chain reaction (PCR), loop-mediated isothermal amplification (LAMP), transcription-mediated amplification (TMA)




Kinetics


Multi-parametric surface plasmon resonance
Dual-polarization interferometry
Quartz crystal microbalance with dissipation monitoring (QCM-D)




Gene function


RNA interference




Other


Bisulfite sequencing
DNA sequencing
Expression cloning
Fluorescence in situ hybridization
Lab-on-a-chip
Comparison of nucleic acid simulation software
Northern blot
Nuclear run-on assay
Radioactivity in the life sciences
Southern blot
Differential centrifugation (sucrose gradient)
Toeprinting assay
Several bioinformatics methods, as seen in list of RNA structure prediction software




See also


CSH Protocols
Current Protocols




References






External links


Protocols for Recombinant DNA Isolation, Cloning, and Sequencing

Kata Kunci Pencarian:

• Ekspresi gen
• Iterbium(III) klorida
• Metabolisme
• Protein
• Rekayasa genetik
• Sejarah kimia
• Pseudogen
• Pangan rekayasa genetika
• Evolusi eksperimental
• Nucleic acid methods
• Nucleic acid
• Nucleic acid quantitation
• Spin column-based nucleic acid purification
• Nucleic acid sequence
• Nucleic acid notation
• Nucleic acid thermodynamics
• Nucleic acid structure
• Nucleic acid analogue
• DNA