Carboxyfluorescein diacetate succinimidyl ester (CFDA-SE) is an amine-reactive, cell-permeable dye generally used in animal cell proliferation research. CFDA-SE is a modified CFSE with two hydroxyl groups on its fluorescein moiety replaced with acetates. This change renders the molecule more hydrophobic and cell-permeable at the expense of its fluorescence property. After entering cells by diffusion, CFDA-SE is cleaved by intracellular esterase enzymes to form CFSE. As its reactive succinimidyl ester group is unmodified, CFDA-SE also covalently binds to lysine residues and other amine sources like CFSE.
If a stained cell divides, the dye is divided equally between the two daughter cells, resulting in both new cells having a CFDA-SE concentration approximately 50% that of the mother cell. A cell stained with CFDA-SE can be kept in culture for several days and fluorescence is detectable in cells following up to 8 successive cell divisions. Fluorescence is typically detected using a flow cytometer on the FL1 detector, with each resulting fluorescent peak representing another round of cell division. The area of each peak is representative of the number of cells in a given division cycle. The staining works best with relatively homogeneous cell populations.
High concentrations of the dye are toxic to animal cells; however, concentrations in the region of 10 micromolar are typically sufficient to give strong staining with minimal cell death. Most cell types will excrete a proportion of the dye over the course of the first 24–48 hours following staining. Dye levels should thereafter remain relatively stable in non-dividing cells.
CFDA-SE is often erroneously referred to as CFSE, even in scientific literature.
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Carboxyfluorescein diacetate succinimidyl ester (CFDA-SE) is an amine-reactive, cell-permeable dye generally used in animal cell proliferation research. CFDA-SE is a modified CFSE with two …
CFDA-SE (5(6)-carboxyfluorescein diacetate succinimidyl ester) is a stable, cell-permeable diacetate precursor to CFSE. Upon diffusion into the cell, intracellular esterases cleave the …
Carboxyfluorescein succinimidyl ester (CFSE) is an effective and popular means to monitor lymphocyte division1-3. CFSE covalently labels long-lived intracellular molecules with the …
Carboxyfluorescein succinimidyl ester (CFSE) is a fluorescent cell staining dye. CFSE is cell permeable and covalently couples, via its succinimidyl group, to intracellular molecules, [1] …
CFDA, SE is a fixable-cell-permeant, fluorescein-based tracer for very long-term cell labeling. The dye is also available in our convenient, easy-to-use Vybrant CFDA-SE Cell Tracer Kit …
Carboxyfluorescein succinimidyl ester (CFSE, 5(6)-Carboxyfluorescein diacetate succinimidyl ester, CFDA-SE, 5(6)-CFDA N-succinmidyl ester) is a fluorescent cell staining dye. CFSE is …
A fluorescent membrane dye (carboxyfluorescein diacetate succinimidyl ester, CFSE) that dilutes with cell division has been used extensively over the last 10 years to provide accurate …
Sep 30, 2024 · CFSE (carboxyfluorescein diacetate succinimidyl ester) is a non-fluorescent dye that can be used to track cell proliferation over multiple generations. It is cell permeable, able …
Carboxyfluorescein succinimidyl ester (CFSE) can be used to measure cell division. In this assay, the parent cell is incubated with CFSE, which is incorporated into DNA during cell division. As …
This protocol outlines the carboxyfluorescein diacetate succinimidyl ester (CFSE) method for following the proliferation of human lymphocytes in vitro and mouse lymphocytes both in vitro …
